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psgrna2 02 pp7 ccdb  (Addgene inc)


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    Structured Review

    Addgene inc psgrna2 02 pp7 ccdb
    Psgrna2 02 Pp7 Ccdb, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/psgrna2+02+pp7+ccdb/psgRNA2%2E02-PP7-ccdB+(Plasmid+%2399912)/pm28849913-122-104-105
    Average 88 stars, based on 1 article reviews
    psgrna2 02 pp7 ccdb - by Bioz Stars, 2026-09
    88/100 stars

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    Modification:

    Article Title: Multiplexed sgRNA Expression Allows Versatile Single Nonrepetitive DNA Labeling and Endogenous Gene Regulation.
    Article Snippet: .. The U6 promoter, 265 conventional and modified sgRNA expression cassettes were amplified from 266 plasmids psgRNA-ccdB (Addgene #: 99915, Supplementary Map 1), 267 psgRNA2.02-MS2-ccdB (Addgene #: 99911, Supplementary Map 2), and 268 Page 13 of 32 ACS Paragon Plus Environment ACS Synthetic Biology 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 14 psgRNA2.02-PP7-ccdB (Addgene #: 99912, Supplementary Map 3). .. The oligos 269 (F + R, 100 μM each) for each targeting sgRNA were phosphorylated by T4 PNK 270 (NEB) and annealed in a thermocycler by using the following parameters: 37 °C 271 for 30 min; 95 °C for 5 min; ramp down to 25 °C at 5 °C min-1.

    Expressing:

    Article Title: Multiplexed sgRNA Expression Allows Versatile Single Nonrepetitive DNA Labeling and Endogenous Gene Regulation.
    Article Snippet: .. The U6 promoter, 265 conventional and modified sgRNA expression cassettes were amplified from 266 plasmids psgRNA-ccdB (Addgene #: 99915, Supplementary Map 1), 267 psgRNA2.02-MS2-ccdB (Addgene #: 99911, Supplementary Map 2), and 268 Page 13 of 32 ACS Paragon Plus Environment ACS Synthetic Biology 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 14 psgRNA2.02-PP7-ccdB (Addgene #: 99912, Supplementary Map 3). .. The oligos 269 (F + R, 100 μM each) for each targeting sgRNA were phosphorylated by T4 PNK 270 (NEB) and annealed in a thermocycler by using the following parameters: 37 °C 271 for 30 min; 95 °C for 5 min; ramp down to 25 °C at 5 °C min-1.

    Amplification:

    Article Title: Multiplexed sgRNA Expression Allows Versatile Single Nonrepetitive DNA Labeling and Endogenous Gene Regulation.
    Article Snippet: .. The U6 promoter, 265 conventional and modified sgRNA expression cassettes were amplified from 266 plasmids psgRNA-ccdB (Addgene #: 99915, Supplementary Map 1), 267 psgRNA2.02-MS2-ccdB (Addgene #: 99911, Supplementary Map 2), and 268 Page 13 of 32 ACS Paragon Plus Environment ACS Synthetic Biology 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 14 psgRNA2.02-PP7-ccdB (Addgene #: 99912, Supplementary Map 3). .. The oligos 269 (F + R, 100 μM each) for each targeting sgRNA were phosphorylated by T4 PNK 270 (NEB) and annealed in a thermocycler by using the following parameters: 37 °C 271 for 30 min; 95 °C for 5 min; ramp down to 25 °C at 5 °C min-1.



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    psgrna2 02 pp7 ccdb  (Addgene inc)
    88
    Addgene inc psgrna2 02 pp7 ccdb
    Psgrna2 02 Pp7 Ccdb, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/psgrna2+02+pp7+ccdb/psgRNA2%2E02-PP7-ccdB+(Plasmid+%2399912)/pm28849913-122-104-105
    Average 88 stars, based on 1 article reviews
    psgrna2 02 pp7 ccdb - by Bioz Stars, 2026-09
    88/100 stars
    		  Buy from Supplier

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